《植物生理学报》 2012, 48(10): 1011-1014

锡金海棠子叶再生体系的建立

吴瑞刚, 杨洪强*, 冉昆, 张玮玮

山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东泰安271018

通信作者：杨洪强；E-mail: hqyang@sdau.edu.cn, labft@sdau.edu.cn；Tel: 0538-8249304

摘　要：

锡金海棠[Malus sikkimensis (Wenz.) Koehne]是我国稀有植物和优良苹果砧木种质资源。本研究探讨了影响锡金海 棠离体再生的条件, 建立了锡金海棠子叶高效离体再生体系。结果表明, 子叶再生体系的最佳诱导培养基为SC+0.1 mg•L-1 NAA+2.0 mg•L-1 TDZ, 最佳分化培养基为SC+0.1 mg•L-1 NAA+1.5 mg•L-1 TDZ, 最佳继代增殖培养基为SC+2.0 mg•L-1 6-BA+0.2 mg•L-1 NAA, 最佳生根培养基为1/2 MS+1.0 mg•L-1 IBA。炼苗后, 移入珍珠岩、花生壳与草炭(1:1:1)的基质中, 20 d移 栽成活率高达90%。

关键词：锡金海棠; 离体再生; 组织培养; 外植体

收稿：2012-09-07   修定：2012-09-24

资助：国家自然科学基金(31171923)、国家转基因生物新品种培 育重大专项(2008ZX08009-3)和高校博士学科点专项科研 基金(20103702110003)。

The Regeneration System Establishment of the Cotyledons of Malus sikkimensis

WU Rui-Gang, YANG Hong-Qiang*, RAN Kun, ZHANG Wei-Wei

College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai’an, Shandong 271018, China

Corresponding author: YANG Hong-Qiang; E-mail: hqyang@sdau.edu.cn, labft@sdau.edu.cn; Tel: 0538-8249304

Abstract:

Malus sikkimensis are rare plants and excellent germplasm resources of apple rootstock in China. In this study, the cotyledons of Malus sikkimensis were used as explants in the investigation of the affective factors of in vitro regeneration conditions and the establishment of efficient regeneration system. The results showed that the best induced medium for callus induction was SC medium with 0.1 mg·L^-1 NAA, 2.0 mg·L^-1 Thidiazuron (TDZ); the best medium for bud differentiation was SC medium with 0.1 mg·L^-1 NAA, 1.5 mg·L^-1 TDZ. The best medium for root differentiation was 1/2 MS medium with 1.0 mg·L^-1 IBA. After acclimatization, the culture-bottle seedlings were transplanted into the matrix, which contains peanut, shells and peat (1:1:1). The survival rate was up to 90%.

Key words: Malus sikkimensis; in vitro regeneration; tissue culture; explants